Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea
Copyright © 2020 Korean Diabetes Association
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
CONFLICTS OF INTEREST
No potential conflict of interest relevant to this article was reported.
AUTHOR CONTRIBUTIONS
Conception or design: E.L., G.R.R., K.H.S.
Acquisition, analysis, or interpretation of data: J.J.K., E.L., G.R.R., S.H.K., Y.B.A., K.H.S.
Drafting the work or revising: J.J.K., K.H.S.
Final approval of the manuscript: J.J.K., E.L., G.R.R., S.H.K., Y.B.A., K.H.S.
Values are presented as absorbance values at 450 nm.
PSC, pancreatic stellate cell; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; IFN-γ, interferon-γ; TNF-α, tumor necrosis factor-α; GM-CSF, granulocyte macrophage-colony stimulating factor; RANTES, regulated on activation normal T cell expressed and secreted; CM, conditioned media.
Samples | IL-1α | IL-1β | IL-2 | IL-4 | IL-6 | IL-10 | IL-12 | IL-13 | IFN-γ | TNF-α | GM-CSF | RANTES |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Negative control | 0.196 | 0.467 | 0.555 | 0.112 | 0.506 | 1.170 | 0.131 | 0.195 | 0.920 | 0.615 | 0.139 | 0.125 |
PSC media (sample 1) | 0.197 | 0.436 | 0.519 | 0.101 | 0.422 | 1.067 | 0.118 | 0.163 | 0.857 | 0.558 | 0.105 | 0.118 |
Normoxia-CM (sample 1) | 0.180 | 0.417 | 0.488 | 0.099 | 0.393 | 1.014 | 0.112 | 0.152 | 0.813 | 0.505 | 0.109 | 0.120 |
Hypoxia-CM (sample 1) | 0.170 | 0.415 | 0.515 | 0.172 | 0.388 | 0.968 | 0.108 | 0.157 | 0.778 | 0.502 | 0.121 | 0.112 |
PSC media (sample 2) | 0.171 | 0.421 | 0.506 | 0.102 | 0.409 | 1.042 | 0.119 | 0.152 | 0.829 | 0.501 | 0.085 | 0.094 |
Normoxia-CM (sample 2) | 0.180 | 0.418 | 0.494 | 0.097 | 0.403 | 1.018 | 0.117 | 0.156 | 0.79 | 0.503 | 0.087 | 0.102 |
Hypoxia-CM (sample 2) | 0.162 | 0.426 | 0.495 | 0.091 | 0.412 | 1.065 | 0.105 | 0.158 | 0.813 | 0.482 | 0.086 | 0.105 |
Positive control | 3.646 | Overflow | Overflow | 0.812 | 0.852 | 3.603 | 2.806 | Overflow | Overflow | Overflow | 2.969 | Overflow |
Samples | IFN-γ | IL-1β | TNF-α |
---|---|---|---|
PSC media (sample 1) | ND | ND | ND |
Normoxia-CM (sample 1) | ND | ND | ND |
Hypoxia-CM (sample 1) | ND | ND | ND |
PSC media (sample 2) | ND | ND | ND |
Normoxia-CM (sample 2) | ND | ND | ND |
Hypoxia-CM (sample 2) | ND | ND | ND |
Values are presented as absorbance values at 450 nm. PSC, pancreatic stellate cell; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; IFN-γ, interferon-γ; TNF-α, tumor necrosis factor-α; GM-CSF, granulocyte macrophage-colony stimulating factor; RANTES, regulated on activation normal T cell expressed and secreted; CM, conditioned media.
The detection limit of cytokines: IFN-γ, 1.1 pg/mL; IL-1β, 80 pg/mL; TNF-α, 25 pg/mL. IL-1β, interleukin-1β; IFN-γ, interferon-γ; TNF-α, tumor necrosis factor-α; PSC, pancreatic stellate cell; ELISA, enzyme-linked immunosorbent assay; ND, not detected; CM, conditioned media.