- Protective Effect of Heme Oxygenase-1 on High Glucose-Induced Pancreatic β-Cell Injury
-
Eun-Mi Lee, Young-Eun Lee, Esder Lee, Gyeong Ryul Ryu, Seung-Hyun Ko, Sung-Dae Moon, Ki-Ho Song, Yu-Bae Ahn
-
Diabetes Metab J. 2011;35(5):469-479. Published online October 31, 2011
-
DOI: https://doi.org/10.4093/dmj.2011.35.5.469
-
-
4,571
View
-
42
Download
-
19
Crossref
-
Abstract
PDFPubReader
- Background
Glucose toxicity that is caused by chronic exposure to a high glucose concentration leads to islet dysfunction and induces apoptosis in pancreatic β-cells. Heme oxygenase-1 (HO-1) has been identified as an anti-apoptotic and cytoprotective gene. The purpose of this study is to investigate whether HO-1 up-regulation when using metalloprotophyrin (cobalt protoporphyrin, CoPP) could protect pancreatic β-cells from high glucose-induced apoptosis. MethodsReverse transcription-polymerase chain reaction was performed to analyze the CoPP-induced mRNA expression of HO-1. Cell viability of INS-1 cells cultured in the presence of CoPP was examined by acridine orange/propidium iodide staining. The generation of intracellular reactive oxygen species (ROS) was measured using flow cytometry. Glucose stimulated insulin secretion (GSIS) was determined following incubation with CoPP in different glucose concentrations. ResultsCoPP increased HO-1 mRNA expression in both a dose- and time-dependent manner. Overexpression of HO-1 inhibited caspase-3, and the number of dead cells in the presence of CoPP was significantly decreased when exposed to high glucose conditions (HG). CoPP also decreased the generation of intracellular ROS by 50% during 72 hours of culture with HG. However, decreased GSIS was not recovered even in the presence of CoPP. ConclusionOur data suggest that CoPP-induced HO-1 up-regulation results in protection from high glucose-induced apoptosis in INS-1 cells; however, glucose stimulated insulin secretion is not restored.
-
Citations
Citations to this article as recorded by
- Diabetes: Risk factor and translational therapeutic implications for Alzheimer's disease
Jeffrey Cummings, Andrew Ortiz, Janelle Castellino, Jefferson Kinney European Journal of Neuroscience.2022; 56(9): 5727. CrossRef - The ischaemic preconditioning paradox and its implications for islet isolation from heart-beating and non heart-beating donors
Daniel Brandhorst, Heide Brandhorst, Samuel Acreman, Paul R. V. Johnson Scientific Reports.2022;[Epub] CrossRef - CYTOCHROMES OF MITOCHONDRIES AND ACTIVITY OF HEME METABOLISM ENZYMES IN THE LIVER UNDER DIFFERENT NUTRIENT REGIMES
G.P. Kopylchuk, Z.-M. I. Grynenkiv, O.M. Voloshchuk Fiziolohichnyĭ zhurnal.2021; 67(2): 37. CrossRef - Heme Oxygenase-1 (HMOX-1) and inhibitor of differentiation proteins (ID1, ID3) are key response mechanisms against iron-overload in pancreatic β-cells
Mawieh Hamad, Abdul Khader Mohammed, Mahmood Y. Hachim, Debasmita Mukhopadhy, Anila Khalique, Amina Laham, Sarah Dhaiban, Khuloud Bajbouj, Jalal Taneera Molecular and Cellular Endocrinology.2021; 538: 111462. CrossRef - Food-derived cyanidin-3-O-glucoside alleviates oxidative stress: evidence from the islet cell line and diabetic db/db mice
Xiang Ye, Wen Chen, Pengcheng Tu, Ruoyi Jia, Yangyang Liu, Yonglu Li, Qiong Tang, Xiaodong Zheng, Qiang Chu Food & Function.2021; 12(22): 11599. CrossRef - Telmisartan protects against high glucose/high lipid‐induced apoptosis and insulin secretion by reducing the oxidative and ER stress
Yan Wang, Jingjing Xue, Yan Li, Xin Zhou, Shun Qiao, Dewu Han Cell Biochemistry and Function.2019; 37(3): 161. CrossRef - Ropivacaine regulates the expression and function of heme oxygenase-1
Xiangbiao Yan, Yu Li, Xuechang Han, Xu Dong, Yajie Zhang, Qunzhi Xing Biomedicine & Pharmacotherapy.2018; 103: 284. CrossRef - A review on heme oxygenase-1 induction: is it a necessary evil
Ajaz Ahmad Waza, Zeenat Hamid, Sajad Ali, Shabir Ahmad Bhat, Musadiq Ahmad Bhat Inflammation Research.2018; 67(7): 579. CrossRef - Diabetes and Alzheimer's Disease: Mechanisms and Nutritional Aspects
Hee Jae Lee, Hye In Seo, Hee Yun Cha, Yun Jung Yang, Soo Hyun Kwon, Soo Jin Yang Clinical Nutrition Research.2018; 7(4): 229. CrossRef - Social, economic, and policy implications of organ preservation advances
Alyssa Ward, David K. Klassen, Kate M. Franz, Sebastian Giwa, Jedediah K. Lewis Current Opinion in Organ Transplantation.2018; 23(3): 336. CrossRef - Geniposide protects pancreatic β cells from high glucose‐mediated injury by activation of AMP‐activated protein kinase
Chunyan Liu, Yanan Hao, Fei Yin, Yonglan Zhang, Jianhui Liu Cell Biology International.2017; 41(5): 544. CrossRef - Eff ects of hemin, a heme oxygenase-1 inducer in L-arginine-induced acute pancreatitis and associated lung injury in adult male albino rats
N. M. Aziz, M. Y. Kamel, R. A. Rifaai Endocrine Regulations.2017; 51(1): 20. CrossRef - Downregulation of microRNA-155 ameliorates high glucose-induced endothelial injury by inhibiting NF-κB activation and promoting HO-1 and NO production
Xi Zhang, Xiangyang Liu, Yang Li, Jingbo Lai, Nana Zhang, Jie Ming, Xianjie Ma, Qiuhe Ji, Ying Xing Biomedicine & Pharmacotherapy.2017; 88: 1227. CrossRef - Sulforaphane Protects against High Cholesterol‐Induced Mitochondrial Bioenergetics Impairments, Inflammation, and Oxidative Stress and Preserves Pancreatic β‐Cells Function
Catalina Carrasco-Pozo, Kah Ni Tan, Martin Gotteland, Karin Borges, Giuseppe Cirillo Oxidative Medicine and Cellular Longevity.2017;[Epub] CrossRef - Aromatic malononitriles stimulate the resistance of insulin-producing beta-cells to oxidants and inflammatory cytokines
Kyril Turpaev, Nils Welsh European Journal of Pharmacology.2016; 784: 69. CrossRef - Differences in insulin biosynthesis pathway between small and large islets do not correspond to insulin secretion
Han-Hung Huang, Lisa Stehno-Bittel Islets.2015; 7(5): e1129097. CrossRef - A newly identified polysaccharide from Ganoderma atrum attenuates hyperglycemia and hyperlipidemia
Kexue Zhu, Shaoping Nie, Chuan Li, Suli Lin, Mengmeng Xing, Wenjuan Li, Deming Gong, Mingyong Xie International Journal of Biological Macromolecules.2013; 57: 142. CrossRef - Glycated Albumin Causes Pancreatic β-Cells Dysfunction Through Autophagy Dysfunction
Young Mi Song, Sun Ok Song, Young-Hye You, Kun-Ho Yoon, Eun Seok Kang, Bong Soo Cha, Hyun Chul Lee, Ji-Won Kim, Byung-Wan Lee Endocrinology.2013; 154(8): 2626. CrossRef - Chronic Resveratrol Treatment Protects Pancreatic Islets against Oxidative Stress in db/db Mice
Young-Eun Lee, Ji-Won Kim, Eun-Mi Lee, Yu-Bae Ahn, Ki-Ho Song, Kun-Ho Yoon, Hyung-Wook Kim, Cheol-Whee Park, Guolian Li, Zhenqi Liu, Seung-Hyun Ko, Kathrin Maedler PLoS ONE.2012; 7(11): e50412. CrossRef
|