- Others
- Increased Nociceptive Responses in Streptozotocin-Induced Diabetic Rats and the Related Expression of Spinal NR2B Subunit of N-Methyl-D-Aspartate Receptors
-
Che Aishah Nazariah Ismail, Rapeah Suppian, Che Badariah Abd Aziz, Khalilah Haris, Idris Long
-
Diabetes Metab J. 2019;43(2):222-235. Published online November 19, 2018
-
DOI: https://doi.org/10.4093/dmj.2018.0020
-
-
5,373
View
-
54
Download
-
14
Web of Science
-
14
Crossref
-
Abstract
PDFPubReader
- Background
This study investigated the role of NR2B in a modulated pain process in the painful diabetic neuropathy (PDN) rat using various pain stimuli. MethodsThirty-two Sprague-Dawley male rats were randomly allocated into four groups (n=8): control, diabetes mellitus (DM) rats and diabetic rats treated with ifenprodil at a lower dose (0.5 µg/day) (I 0.5) or higher dose (1.0 µg/day) (I 1.0). DM was induced by a single injection of streptozotocin at 60 mg/kg on day 0 of experimentation. Diabetic status was assessed on day 3 of the experimentation. The responses on both tactile and thermal stimuli were assessed on day 0 (baseline), day 14 (pre-intervention), and day 22 (post-intervention). Ifenprodil was given intrathecally for 7 days from day 15 until day 21. On day 23, 5% formalin was injected into the rats' hind paw and the nociceptive responses were recorded for 1 hour. The rats were sacrificed 72 hours post-formalin injection and an analysis of the spinal NR2B expression was performed. ResultsDM rats showed a significant reduction in pain threshold in response to the tactile and thermal stimuli and higher nociceptive response during the formalin test accompanied by the higher expression of phosphorylated spinal NR2B in both sides of the spinal cord. Ifenprodil treatment for both doses showed anti-allodynic and anti-nociceptive effects with lower expression of phosphorylated and total spinal NR2B. ConclusionWe suggest that the pain process in the streptozotocin-induced diabetic rat that has been modulated is associated with the higher phosphorylation of the spinal NR2B expression in the development of PDN, which is similar to other models of neuropathic rats.
-
Citations
Citations to this article as recorded by
- Painful diabetic neuropathy: The role of ion channels
Qi Wang, Yifei Ye, Linghui Yang, Lifan Xiao, Jin Liu, Wensheng Zhang, Guizhi Du Biomedicine & Pharmacotherapy.2024; 173: 116417. CrossRef - Enantiomerically Pure Indazole Bioisosteres of Ifenprodil and Ro 25-6981 as Negative Allosteric Modulators of NMDA Receptors with the GluN2B Subunit
Judith Lüken, Gunnar Goerges, Julian A. Schreiber, Judith Schmidt, Bastian Frehland, Dirk Schepmann, Guiscard Seebohm, Bernhard Wünsch Journal of Medicinal Chemistry.2024;[Epub] CrossRef - Therapeutic potential of progesterone in spinal cord injury‐induced neuropathic pain: At the crossroads between neuroinflammation and N‐methyl‐D‐aspartate receptor
Sol Ferreyra, Susana González Journal of Neuroendocrinology.2023;[Epub] CrossRef - Chemical, pharmacodynamic and pharmacokinetic characterization of the GluN2B receptor antagonist 3-(4-phenylbutyl)-2,3,4,5-tetrahydro-1H-3-benzazepine-1,7-diol – starting point for PET tracer development
Marvin Korff, Ruben Steigerwald, Elena Bechthold, Dirk Schepmann, Julian A. Schreiber, Sven G. Meuth, Guiscard Seebohm, Bernhard Wünsch Biological Chemistry.2023; 404(4): 279. CrossRef - Mechanisms of Transmission and Processing of Pain: A Narrative Review
Girolamo Di Maio, Ines Villano, Ciro Rosario Ilardi, Antonietta Messina, Vincenzo Monda, Ashlei Clara Iodice, Chiara Porro, Maria Antonietta Panaro, Sergio Chieffi, Giovanni Messina, Marcellino Monda, Marco La Marra International Journal of Environmental Research and Public Health.2023; 20(4): 3064. CrossRef - Indazole as a Phenol Bioisostere: Structure–Affinity Relationships of GluN2B-Selective NMDA Receptor Antagonists
Judith Lüken, Gunnar Goerges, Nadine Ritter, Paul Disse, Julian A. Schreiber, Judith Schmidt, Bastian Frehland, Dirk Schepmann, Guiscard Seebohm, Bernhard Wünsch Journal of Medicinal Chemistry.2023; 66(16): 11573. CrossRef - Synthesis of oxazolo‐annulated 3‐benzazepines designed by merging two negative allosteric NMDA receptor modulators
Alexander Markus, Dirk Schepmann, Bernhard Wünsch Archiv der Pharmazie.2022;[Epub] CrossRef - Phenol—Benzoxazolone bioisosteres: Synthesis and biological evaluation of tricyclic GluN2B‐selective N‐methyl‐d‐aspartate receptor antagonists
Alexander Markus, Julian A. Schreiber, Gunnar Goerges, Bastian Frehland, Guiscard Seebohm, Dirk Schepmann, Bernhard Wünsch Archiv der Pharmazie.2022;[Epub] CrossRef - Ifenprodil Reduced Expression of Activated Microglia, BDNF and DREAM Proteins in the Spinal Cord Following Formalin Injection During the Early Stage of Painful Diabetic Neuropathy in Rats
Che Aishah Nazariah Ismail, Rapeah Suppian, Che Badariah Ab Aziz, Idris Long Journal of Molecular Neuroscience.2021; 71(2): 379. CrossRef - Ifenprodil Stereoisomers: Synthesis, Absolute Configuration, and Correlation with Biological Activity
Elena Bechthold, Julian A. Schreiber, Kirstin Lehmkuhl, Bastian Frehland, Dirk Schepmann, Freddy A. Bernal, Constantin Daniliuc, Inés Álvarez, Cristina Val Garcia, Thomas J. Schmidt, Guiscard Seebohm, Bernhard Wünsch Journal of Medicinal Chemistry.2021; 64(2): 1170. CrossRef - The anti-diabetic effects of betanin in streptozotocin-induced diabetic rats through modulating AMPK/SIRT1/NF-κB signaling pathway
Nasim Abedimanesh, Somayyeh Asghari, Kosar Mohammadnejad, Zahra Daneshvar, Soudeh Rahmani, Samaneh Shokoohi, Amir Hasan Farzaneh, Seyed Hojjat Hosseini, Iraj Jafari Anarkooli, Maryam Noubarani, Sina Andalib, Mohammad Reza Eskandari, Behrooz Motlagh Nutrition & Metabolism.2021;[Epub] CrossRef - Diabetic neuropathy and neuropathic pain: a (con)fusion of pathogenic mechanisms?
Nigel A. Calcutt Pain.2020; 161(Supplement): S65. CrossRef - N-Acetylcysteine causes analgesia in a mouse model of painful diabetic neuropathy
Serena Notartomaso, Pamela Scarselli, Giada Mascio, Francesca Liberatore, Emanuela Mazzon, Santa Mammana, Agnese Gugliandolo, Giorgio Cruccu, Valeria Bruno, Ferdinando Nicoletti, Giuseppe Battaglia Molecular Pain.2020;[Epub] CrossRef - Effect of aerobic exercise on innate immune responses and inflammatory mediators in the spinal cord of diabetic rats
A. Kaki, M. Nikbakht, A.H. Habibi, H.F. Moghadam Comparative Exercise Physiology.2020; 16(4): 293. CrossRef
|