- Type 1 Diabetes
- Differential Profile of Plasma Circular RNAs in Type 1 Diabetes Mellitus
-
Yangyang Li, Ying Zhou, Minghui Zhao, Jing Zou, Yuxiao Zhu, Xuewen Yuan, Qianqi Liu, Hanqing Cai, Cong-Qiu Chu, Yu Liu
-
Diabetes Metab J. 2020;44(6):854-865. Published online July 13, 2020
-
DOI: https://doi.org/10.4093/dmj.2019.0151
-
-
6,875
View
-
151
Download
-
23
Web of Science
-
23
Crossref
-
 Abstract
 PDF Supplementary Material PubReader  ePub
-
Background
No currently available biomarkers or treatment regimens fully meet therapeutic needs of type 1 diabetes mellitus (T1DM). Circular RNA (circRNA) is a recently identified class of stable noncoding RNA that have been documented as potential biomarkers for various diseases. Our objective was to identify and analyze plasma circRNAs altered in T1DM.
Methods
We used microarray to screen differentially expressed plasma circRNAs in patients with new onset T1DM (n=3) and age-/gender-matched healthy controls (n=3). Then, we selected six candidates with highest fold-change and validated them by quantitative real-time polymerase chain reaction in independent human cohort samples (n=12). Bioinformatic tools were adopted to predict putative microRNAs (miRNAs) sponged by these validated circRNAs and their downstream messenger RNAs (mRNAs). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to gain further insights into T1DM pathogenesis.
Results
We identified 68 differentially expressed circRNAs, with 61 and seven being up- and downregulated respectively. Four of the six selected candidates were successfully validated. Curations of their predicted interacting miRNAs revealed critical roles in inflammation and pathogenesis of autoimmune disorders. Functional relations were visualized by a circRNA-miRNA-mRNA network. GO and KEGG analyses identified multiple inflammation-related processes that could be potentially associated with T1DM pathogenesis, including cytokine-cytokine receptor interaction, inflammatory mediator regulation of transient receptor potential channels and leukocyte activation involved in immune response.
Conclusion
Our study report, for the first time, a profile of differentially expressed plasma circRNAs in new onset T1DM. Further in silico annotations and bioinformatics analyses supported future application of circRNAs as novel biomarkers of T1DM.
-
Citations
Citations to this article as recorded by  - Non-coding RNAs and exosomal non-coding RNAs in diabetic retinopathy: A narrative review
Yuhong Zhong, Juan Xia, Li Liao, Mohammad Reza Momeni
International Journal of Biological Macromolecules.2024; 259: 128182. CrossRef - Circular RNAs: Potential biomarkers and therapeutic targets for autoimmune diseases
Ren-Jie Zhao, Wan-Ying Zhang, Xing-Xing Fan
Heliyon.2024; 10(1): e23694. CrossRef - Hsa_circRNA_405498 and hsa_circRNA_100033 Serve as Potential Biomarkers for Differential Diagnosis of Type 1 Diabetes
Ziwei Zhang, Shuoming Luo, Zilin Xiao, Wenfeng Yin, Xiajie Shi, Hongzhi Chen, Zhiguo Xie, Zhenqi Liu, Xia Li, Zhiguang Zhou
The Journal of Clinical Endocrinology & Metabolism.2024; 109(6): 1464. CrossRef - A Comprehensive Review on Circulating cfRNA in Plasma: Implications for Disease Diagnosis and Beyond
Pengqiang Zhong, Lu Bai, Mengzhi Hong, Juan Ouyang, Ruizhi Wang, Xiaoli Zhang, Peisong Chen
Diagnostics.2024; 14(10): 1045. CrossRef - Circulating non-coding RNA in type 1 diabetes mellitus as a source of potential biomarkers – An emerging role of sex difference
Lucyna Stachowiak, Weronika Kraczkowska, Aleksandra Świercz, Paweł Piotr Jagodziński
Biochemical and Biophysical Research Communications.2024; 736: 150482. CrossRef - Importance of Studying Non-Coding RNA in Children and Adolescents with Type 1 Diabetes
Manuela Cabiati, Giovanni Federico, Silvia Del Ry
Biomedicines.2024; 12(9): 1988. CrossRef - Circular RNAs in human diseases
Yuanyong Wang, Jin Zhang, Yuchen Yang, Zhuofeng Liu, Sijia Sun, Rui Li, Hui Zhu, Tian Li, Jin Zheng, Jie Li, Litian Ma
MedComm.2024;[Epub] CrossRef - Research progress of circular RNA molecules in aging and age-related diseases
Zhidan Zhang, Yuling Huang, AYao Guo, Lina Yang
Ageing Research Reviews.2023; 87: 101913. CrossRef - CircRNAs and RNA-Binding Proteins Involved in the Pathogenesis of Cancers or Central Nervous System Disorders
Yuka Ikeda, Sae Morikawa, Moeka Nakashima, Sayuri Yoshikawa, Kurumi Taniguchi, Haruka Sawamura, Naoko Suga, Ai Tsuji, Satoru Matsuda
Non-Coding RNA.2023; 9(2): 23. CrossRef - Decrypting the circular RNAs does a favor for us: Understanding, diagnosing and treating diabetes mellitus and its complications
Zi Li, Yuanyuan Ren, Ziwei Lv, Man Li, Yujia Li, Xiaobin Fan, Yuyan Xiong, Lu Qian
Biomedicine & Pharmacotherapy.2023; 168: 115744. CrossRef - Circular RNA PIP5K1A Promotes Glucose and Lipid Metabolism Disorders and Inflammation in Type 2 Diabetes Mellitus
Ge Song, YiQian Zhang, YiHua Jiang, Huan Zhang, Wen Gu, Xiu Xu, Jing Yao, ZhengFang Chen
Molecular Biotechnology.2023;[Epub] CrossRef - Circular RNA PIP5K1A act as microRNA-552-3p sponge to regulates inflammation, oxidative damage in glucolipotoxicity-induced pancreatic INS-1 β-cells via Janus kinase 1
Lei Ren
Bioengineered.2022; 13(3): 5724. CrossRef - Circular RNAs in diabetes mellitus and its complications
Wenqi Fan, Haipeng Pang, Zhiguo Xie, Gan Huang, Zhiguang Zhou
Frontiers in Endocrinology.2022;[Epub] CrossRef - Type 1 Diabetes Mellitus-Related circRNAs Regulate CD4+ T Cell Functions
Jianni Chen, Guanfei Jia, Xue Lv, Shufa Li, Christos K. Kontos
BioMed Research International.2022; 2022: 1. CrossRef - An intriguing role of circular RNA in insulin resistance and endothelial dysfunction: the future perspectives
Monisha Prasad, Selvaraj Jayaraman, Vishnu Priya Veeraraghavan
Hypertension Research.2022; 45(11): 1843. CrossRef - Circular RNAs in Diabetic Nephropathy: Updates and Perspectives
Miao Liu, Junli Zhao
Aging and disease.2022; 13(5): 1365. CrossRef - CircRNAs: Key molecules in the prevention and treatment of ischemic stroke
Zeyu Liu, Yanhong Zhou, Jian Xia
Biomedicine & Pharmacotherapy.2022; 156: 113845. CrossRef - Pro-Inflammatory Cytokines Promote the Transcription of Circular RNAs in Human Pancreatic β Cells
Simranjeet Kaur, Caroline Frørup, Aashiq H. Mirza, Tina Fløyel, Reza Yarani, Maikel L. Colli, Jesper Johannesen, Joachim Størling, Decio L. Eizirik, Flemming Pociot
Non-Coding RNA.2022; 8(5): 69. CrossRef - Differential Expression and Bioinformatics Analysis of Plasma-Derived Exosomal circRNA in Type 1 Diabetes Mellitus
Haipeng Pang, Wenqi Fan, Xiajie Shi, Shuoming Luo, Yimeng Wang, Jian Lin, Yang Xiao, Xia Li, Gan Huang, Zhiguo Xie, Zhiguang Zhou, Jinhui Liu
Journal of Immunology Research.2022; 2022: 1. CrossRef - Circular RNAs in diabetes and its complications: Current knowledge and future prospects
Wenfeng Yin, Ziwei Zhang, Zilin Xiao, Xia Li, Shuoming Luo, Zhiguang Zhou
Frontiers in Genetics.2022;[Epub] CrossRef - Circular RNA in autoimmune diseases: special emphasis on regulation mechanism in RA and SLE
Yurong Huang, Qiuyun Xue, Chenglong Cheng, Yuting Wang, Xiao Wang, Jun Chang, Chenggui Miao
Journal of Pharmacy and Pharmacology.2022;[Epub] CrossRef - Emerging roles of circular RNAs in systemic lupus erythematosus
Xin Wang, Rui Ma, Weimin Shi, Zhouwei Wu, Yuling Shi
Molecular Therapy - Nucleic Acids.2021; 24: 212. CrossRef - Understanding Competitive Endogenous RNA Network Mechanism in Type 1 Diabetes Mellitus Using Computational and Bioinformatics Approaches
Xuanzi Yi, Xu Cheng
Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy.2021; Volume 14: 3865. CrossRef
|
KDA
