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Norman Balcazar  (Balcazar N) 1 Article
Obesity and Metabolic Syndrome
In Vitro Effect of Fatty Acids Identified in the Plasma of Obese Adolescents on the Function of Pancreatic β-Cells
Claudia Velasquez, Juan Sebastian Vasquez, Norman Balcazar
Diabetes Metab J. 2017;41(4):303-315.   Published online May 24, 2017
DOI: https://doi.org/10.4093/dmj.2017.41.4.303
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  • 8 Web of Science
  • 6 Crossref
AbstractAbstract PDFPubReader   
Background

The increase in circulating free fatty acid (FFA) levels is a major factor that induces malfunction in pancreatic β-cells. We evaluated the effect of FFAs reconstituted according to the profile of circulating fatty acids found in obese adolescents on the viability and function of the murine insulinoma cell line (mouse insulinoma [MIN6]).

Methods

From fatty acids obtained commercially, plasma-FFA profiles of three different youth populations were reconstituted: obese with metabolic syndrome; obese without metabolic syndrome; and normal weight without metabolic syndrome. MIN6 cells were treated for 24 or 48 hours with the three FFA profiles, and glucose-stimulated insulin secretion, cell viability, mitochondrial function and antioxidant activity were evaluated.

Results

The high FFA content and high polyunsaturated ω6/ω3 ratio, present in plasma of obese adolescents with metabolic syndrome had a toxic effect on MIN6 cell viability and function, increasing oxidative stress and decreasing glucose-dependent insulin secretion.

Conclusion

These results could help to guide nutritional management of obese young individuals, encouraging the increase of ω-3-rich food consumption in order to reduce the likelihood of deterioration of β-cells and the possible development of type 2 diabetes mellitus.

Citations

Citations to this article as recorded by  
  • The reversible effects of free fatty acids on sulfonylurea-stimulated insulin secretion are related to the expression and dynamin-mediated endocytosis of KATP channels in pancreatic β cells
    Chenmin Wei, Zichen Zhang, Qi Fu, Yunqiang He, Tao Yang, Min Sun
    Endocrine Connections.2023;[Epub]     CrossRef
  • Preparation of fatty acid solutions exerts significant impact on experimental outcomes in cell culture models of lipotoxicity
    Axel Römer, Divya Rawat, Thomas Linn, Sebastian F Petry
    Biology Methods and Protocols.2022;[Epub]     CrossRef
  • Lipotoxic Impairment of Mitochondrial Function in β-Cells: A Review
    Axel Römer, Thomas Linn, Sebastian F. Petry
    Antioxidants.2021; 10(2): 293.     CrossRef
  • Contribution of Oxidative Stress and Impaired Biogenesis of Pancreatic β-Cells to Type 2 Diabetes
    Petr Ježek, Martin Jabůrek, Lydie Plecitá-Hlavatá
    Antioxidants & Redox Signaling.2019; 31(10): 722.     CrossRef
  • The role of polyunsaturated fatty acids (n-3 PUFAs) on the pancreatic β-cells and insulin action
    Habtamu Wondifraw Baynes, Seifu Mideksa, Sintayehu Ambachew
    Adipocyte.2018; : 1.     CrossRef
  • Fatty Acid-Stimulated Insulin Secretion vs. Lipotoxicity
    Petr Ježek, Martin Jabůrek, Blanka Holendová, Lydie Plecitá-Hlavatá
    Molecules.2018; 23(6): 1483.     CrossRef

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